Volume 15, Issue 3 (Paramedical Sciences and Military Health (Autumn 2020) 2020)
Paramedical Sciences and Military Health 2020, 15(3): 1-7 |
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babaei K, Rasouli A. Evaluation of Antibacterial Properties of Different Concentrations of Pecanum Harmala Ethanolic Extract on Some Standard Gram-Positive and Gram-Negative Bacteria in Vitro. Paramedical Sciences and Military Health 2020; 15 (3) :1-7
URL: http://jps.ajaums.ac.ir/article-1-238-en.html
URL: http://jps.ajaums.ac.ir/article-1-238-en.html
1- Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran , khaterehbabaei2021@gmail.com
2- Department of Biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran
2- Department of Biology, Islamic Azad University, Tabriz Branch, Tabriz, Iran
Abstract: (1506 Views)
Introduction: Today, due to the increased drug resistance of bacteria to common antibiotics, it is important to find new antimicrobials with easy access, natural and with fewer side effects. The aim of this study was to investigate the effect of Pecanum harmala ethanolic extract on the activity of some standard bacteria in vitro.
Methods and Materials: After collecting the plants and confirming their scientific name, Pecanum harmala extract was extracted by Soxhlet extractor distillation method and different concentrations of the extract were prepared. Then Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) extracts of standard bacteria of Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa were determined by dilution in broth and well propagation in agar. The above test has been repeated 5 times to decrease the test error. Data analyses were performed using SPSS software version 18
Results: The results showed that Pecanum harmala ethanolic extract inhibits the growth of gram-positive bacteria compared to gram-negative bacteria in high concentrations.
Conclusion: The results of this study show that Pecanum harmala can be considered as a group of medicinal plants with antibacterial properties. After evaluating their effects in vivo and identifying the active ingredients, it can be used as an alternative to conventional chemical drugs in used to treat infections.
Methods and Materials: After collecting the plants and confirming their scientific name, Pecanum harmala extract was extracted by Soxhlet extractor distillation method and different concentrations of the extract were prepared. Then Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) extracts of standard bacteria of Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa were determined by dilution in broth and well propagation in agar. The above test has been repeated 5 times to decrease the test error. Data analyses were performed using SPSS software version 18
Results: The results showed that Pecanum harmala ethanolic extract inhibits the growth of gram-positive bacteria compared to gram-negative bacteria in high concentrations.
Conclusion: The results of this study show that Pecanum harmala can be considered as a group of medicinal plants with antibacterial properties. After evaluating their effects in vivo and identifying the active ingredients, it can be used as an alternative to conventional chemical drugs in used to treat infections.
Type of Study: Research |
Subject:
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Received: 2021/01/18 | Accepted: 2021/02/18 | Published: 2020/12/20
Received: 2021/01/18 | Accepted: 2021/02/18 | Published: 2020/12/20
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