Volume 12, Issue 4 (Paramedical Sciences and Military Health (winter 2018) 2017)
Paramedical Sciences and Military Health 2017, 12(4): 9-15 |
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sadeghi V, amini K. The Molecular Prevalence of stx1, stx2 and eaeA and hly Genes from Escherichia Coli Isolated from Fecal Specimens of Rafsanjan Health Centers Using Multiplex PCR. Paramedical Sciences and Military Health 2017; 12 (4) :9-15
URL: http://jps.ajaums.ac.ir/article-1-121-en.html
URL: http://jps.ajaums.ac.ir/article-1-121-en.html
1- Department of Microbiology, Faculty of Basic Sciences, Sirjan Branch, Islamic Azad University, Sirjan, Iran.
2- Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran. ,dr_kumarss_amini@yahoo.com
2- Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran. ,
Abstract: (3571 Views)
Introduction: Escherichia coli is a normal flora of warm-blood animals digestive tract. Most strains of bacteria are not pathogenic; however, some strains can cause a variety of diseases. Intestinal and extraintestinal human infections caused by the contaminated food and water. The present study aimed to investigate the molecular genes stx1, stx2, eae A, hly of Escherichia coli isolated from human sources by Multiplex PCR method.
Methods and Materials: 55 fecal samples were collected from patients having diarrhoea symptoms in Ali ibn Abitalib hospital and several private medical diagnostic labratories in Rafsanjan. After enriching and isolating the bacteria and extracting DNA, Stx1, Stx2, eaeA, hly genes were evaluated by multiplex PCR method.
Results: Based on the results of molecular analysis of 55 human samples, 10 samples (18.1%) Stx2 gene and 4 (5.45%) Stx1 gene were identified. Other genes of eaeA and hly were not detected in any of the samples.
Discussion and Conclusion: Detection of Escherichia coli strains using molecular methods can be accomplished quickly and accurately. Moreover, considering these genes and the profile of antibiotics is recommended in various other sources.
Methods and Materials: 55 fecal samples were collected from patients having diarrhoea symptoms in Ali ibn Abitalib hospital and several private medical diagnostic labratories in Rafsanjan. After enriching and isolating the bacteria and extracting DNA, Stx1, Stx2, eaeA, hly genes were evaluated by multiplex PCR method.
Results: Based on the results of molecular analysis of 55 human samples, 10 samples (18.1%) Stx2 gene and 4 (5.45%) Stx1 gene were identified. Other genes of eaeA and hly were not detected in any of the samples.
Discussion and Conclusion: Detection of Escherichia coli strains using molecular methods can be accomplished quickly and accurately. Moreover, considering these genes and the profile of antibiotics is recommended in various other sources.
Type of Study: Research |
Subject:
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Received: 2017/11/24 | Accepted: 2018/04/9 | Published: 2018/05/7
Received: 2017/11/24 | Accepted: 2018/04/9 | Published: 2018/05/7
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