Volume 15, Issue 1 (Paramedical Sciences and Military Health (Spring 2020) 2020)
Paramedical Sciences and Military Health 2020, 15(1): 26-34 |
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- F G, - A K, - A D G, - P T, - M B, - A R, et al . Anti-Toxoplasma Effects of Artemisia aucheri Extract in Vitro. Paramedical Sciences and Military Health 2020; 15 (1) :26-34
URL: http://jps.ajaums.ac.ir/article-1-216-en.html
URL: http://jps.ajaums.ac.ir/article-1-216-en.html
Fatemeh Ghaffarifar - 
1, Amir KarimiPourSaryazdi -2 , Ali Dalir Ghaffari -2 , Pooya Tavakoli -2 , Mohammad Barati - , Aliakbar Rasekhi -3 , Yeganeh KarimiPourSaryazdi -4 , Bahareh Siahkohy -5
1, Amir KarimiPourSaryazdi -2 , Ali Dalir Ghaffari -2 , Pooya Tavakoli -2 , Mohammad Barati - , Aliakbar Rasekhi -3 , Yeganeh KarimiPourSaryazdi -4 , Bahareh Siahkohy -5
1- Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , ghafarif@modares.ac.ir
2- Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3- 3. Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4- 4. Faculty of medicine, Tehran medical science, Islamic Azad university, Tehran, Iran
5- 5. Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
2- Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3- 3. Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4- 4. Faculty of medicine, Tehran medical science, Islamic Azad university, Tehran, Iran
5- 5. Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Abstract: (2321 Views)
Introduction: Toxoplasma gondii (T. gondii) is a compulsive intracellular parasite of the Apicomplexa phylum that contaminates a wide range of vertebrates including humans, and it causes disease. The current study aimed to evaluate the anti-Toxoplasma gondii effects of Artemisia aucheri.
Methods and Materials: Tachyzoite assay was performed to evaluate the death rate of the parasite. Flow cytometry technique has been used in order to determine the possible induction of apoptosis in T. gondii tachyzoites. Furthermore, 50% inhibitory concentration (IC50) was obtained for macrophages by MTT assay.
Results: The IC50 values of autumn and spring extracts of A. aucheri was estimated about 136 μg/ml and 119 μg/ml, respectively, in treated T. gondii tachyzoites. After 24 hours of macrophage incubation, the highest rate of cytotoxicity was observed in high concentrations of spring and autumn A. aucheri extracts. Based on the flow cytometry results the rates of normal, necrotic, and apoptotic cells treated with autumn extract were 93.1%, 2.45%, and 4.49%, respectively, and when with spring extract was treated, the rates were 60.4%, 3.99%, and 35.54%, respectively.
Discussion and Conclusion: We concluded that the autumn and spring extracts of A. aucheri has lethal effect on T. gondii tachyzoites and also induces the apoptosis in parasites.
Methods and Materials: Tachyzoite assay was performed to evaluate the death rate of the parasite. Flow cytometry technique has been used in order to determine the possible induction of apoptosis in T. gondii tachyzoites. Furthermore, 50% inhibitory concentration (IC50) was obtained for macrophages by MTT assay.
Results: The IC50 values of autumn and spring extracts of A. aucheri was estimated about 136 μg/ml and 119 μg/ml, respectively, in treated T. gondii tachyzoites. After 24 hours of macrophage incubation, the highest rate of cytotoxicity was observed in high concentrations of spring and autumn A. aucheri extracts. Based on the flow cytometry results the rates of normal, necrotic, and apoptotic cells treated with autumn extract were 93.1%, 2.45%, and 4.49%, respectively, and when with spring extract was treated, the rates were 60.4%, 3.99%, and 35.54%, respectively.
Discussion and Conclusion: We concluded that the autumn and spring extracts of A. aucheri has lethal effect on T. gondii tachyzoites and also induces the apoptosis in parasites.
Type of Study: Research |
Subject:
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Received: 2020/06/28 | Accepted: 2020/06/19 | Published: 2020/06/19
Received: 2020/06/28 | Accepted: 2020/06/19 | Published: 2020/06/19
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