Volume 19, Issue 3 (Paramedical Sciences and Military Health (Autumn 2024) 2024)
Paramedical Sciences and Military Health 2024, 19(3): 1-6 |
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Norouzi norouzmahalleh M, Vahidi M, Yadegari M H, saraf Z. Investigating the Effect of Beta-Glucan Extracted from Saccharomyces cerevisiae on Tumor Growth and Apoptosis Induction in CT26 Murine Colon Cancer Cell Line. Paramedical Sciences and Military Health 2024; 19 (3) :1-6
URL: http://jps.ajaums.ac.ir/article-1-445-en.html
URL: http://jps.ajaums.ac.ir/article-1-445-en.html
1- Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. , meisam.nowruzi@gmail.com
2- Department of Medical Laboratory Sciences, School of Allied Medical Sciences, AJA University of Medical Sciences, Tehran, Iran.
3- Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4- Department of Medical Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
2- Department of Medical Laboratory Sciences, School of Allied Medical Sciences, AJA University of Medical Sciences, Tehran, Iran.
3- Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4- Department of Medical Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Abstract: (90 Views)
Introduction: Colon cancer is a type of cancer that develops in the tissues of the large intestine and it is one of the most common cancers worldwide. Generally, most chemotherapy drugs have significant side effects; therefore, researchers are seeking bioactive compounds to enhance the immune system and tumor defense mechanisms.
Materials and Methods: The effects of β-glucan extracted from Saccharomyces cerevisiae on the growth and apoptosis of the CT26 murine colon cancer cell line were evaluated in this study,. CT26 tumor cells were cultured in DMEM/F12 medium supplemented with 10% fetal bovine serum, 1% L-glutamine, and 1% penicillin-streptomycin and they incubated at 37°C with
5% CO₂. After 24 hours, the cells were treated with different concentrations of β-glucan for 48 hours, and cell viability was assessed using the MTT assay. To confirm the extent of cell death, apoptosis was analyzed using an Annexin V/PI apoptosis detection kit and flow cytometry.
Results: The results indicated that β-glucan at a concentration of 700 μg/ml significantly inhibited the growth of cancer cells in vitro and it induced apoptosis.
Conclusion: Based on these findings, β-glucan appears to be a promising bioactive compound for reducing cancer cell proliferation in future studies.
Materials and Methods: The effects of β-glucan extracted from Saccharomyces cerevisiae on the growth and apoptosis of the CT26 murine colon cancer cell line were evaluated in this study,. CT26 tumor cells were cultured in DMEM/F12 medium supplemented with 10% fetal bovine serum, 1% L-glutamine, and 1% penicillin-streptomycin and they incubated at 37°C with
5% CO₂. After 24 hours, the cells were treated with different concentrations of β-glucan for 48 hours, and cell viability was assessed using the MTT assay. To confirm the extent of cell death, apoptosis was analyzed using an Annexin V/PI apoptosis detection kit and flow cytometry.
Results: The results indicated that β-glucan at a concentration of 700 μg/ml significantly inhibited the growth of cancer cells in vitro and it induced apoptosis.
Conclusion: Based on these findings, β-glucan appears to be a promising bioactive compound for reducing cancer cell proliferation in future studies.
Type of Study: Research |
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Received: 2024/07/2 | Accepted: 2024/08/4 | Published: 2024/09/26
Received: 2024/07/2 | Accepted: 2024/08/4 | Published: 2024/09/26
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